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KMID : 0379119980260020275
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Korean Journal of Mycology
1998 Volume.26 No. 2 p.275 ~ p.280
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Protoplasts Isolation and Reversion of Fomitella fraxinea
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김경수/Kim, Kyung Soo
김영호/차동열/유창현/공원식/Kim, Young Ho/Cha, Dong Yeul/You, Chang Hyun/Kong, Won Sik
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Abstract
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Factors affecting protoplasts isolation and regeneration of Fomitella fraxinea were investigated. Lytic enzyme mixture of Novozym 234, Cellulase onozuka R-10 and β-Glucuronidase was found to be the best for the protoplasts isolation. Osmotic stabilizer of 0.6 M sucrose was observed as the best for protoplasts isolation. The highest number of protoplasts was obtained from the F. fraxinea mycelium with lytic enzyme mixture and osmotic stabilizer that had been cultured for 3 hours. The highest regeneration rate of 0.02% was achieved when the 0.6 M sorbitol was employed as osmotic stabilizer.
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